<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:f723486e-8cb1-4bbc-ba45-be59f38aa3bd,g:7a08eedb-b70a-4d2d-ae81-88fd2315977f-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="aqst-20220930.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_f86cb873-9a92-4332-8c82-25c2fbfae1ab" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_us-gaap_AccountsPayableCurrent_f86cb873-9a92-4332-8c82-25c2fbfae1ab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansPayableCurrent_a48c1795-c31b-4405-a093-4fb2aa9303ff" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LoansPayableCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_us-gaap_LoansPayableCurrent_a48c1795-c31b-4405-a093-4fb2aa9303ff" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_20307533-1a0b-4ff5-b109-9c346e4fdf35" xlink:href="aqst-20220930.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_20307533-1a0b-4ff5-b109-9c346e4fdf35" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_f93304cb-b26b-46a0-bca2-eaa19abfdc42" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_f93304cb-b26b-46a0-bca2-eaa19abfdc42" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_ac6e48cf-b87e-47d3-81c0-f50cbfd1b2e0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_ac6e48cf-b87e-47d3-81c0-f50cbfd1b2e0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_a1ee433a-3385-49d5-8d2f-2acd6faf4f52" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_88a8b950-0cfb-4c04-99c3-8ed92900a8a5" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_a1ee433a-3385-49d5-8d2f-2acd6faf4f52" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_6e188716-f6bd-4d08-935d-6ffd39665edf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_6b6d2452-c17f-47e5-80fd-7810bce4e320" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_6e188716-f6bd-4d08-935d-6ffd39665edf" xlink:to="loc_us-gaap_CommonStockValue_6b6d2452-c17f-47e5-80fd-7810bce4e320" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_9acb84fa-6dab-472d-bc81-c3f60c05bf4b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_6e188716-f6bd-4d08-935d-6ffd39665edf" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_9acb84fa-6dab-472d-bc81-c3f60c05bf4b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_8df43dcd-776a-4bd8-9956-29ac3d164b6d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_6e188716-f6bd-4d08-935d-6ffd39665edf" xlink:to="loc_us-gaap_AdditionalPaidInCapital_8df43dcd-776a-4bd8-9956-29ac3d164b6d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_c8034b9d-618c-4e03-a925-d89b643578a4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_c8034b9d-618c-4e03-a925-d89b643578a4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_7c693e0f-6532-4748-b95e-778830afe222" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_7c693e0f-6532-4748-b95e-778830afe222" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_53111c8c-7da5-4179-9160-883dabc56b25" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:to="loc_us-gaap_AssetsCurrent_53111c8c-7da5-4179-9160-883dabc56b25" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_dc345aaf-9a33-4471-b0ca-e41d20bb99f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_dc345aaf-9a33-4471-b0ca-e41d20bb99f9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_8de138c6-a819-435a-91ad-d8fb842c0751" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_1a185662-e6f9-46c1-a8c7-86b3fb0cae43" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_8de138c6-a819-435a-91ad-d8fb842c0751" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_28092213-c991-45a0-ae00-e234b7f2b724" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_c40808f1-aa4e-4a22-8a65-71d8009115cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_28092213-c991-45a0-ae00-e234b7f2b724" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_c40808f1-aa4e-4a22-8a65-71d8009115cc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_461f69f4-e080-486c-9bd3-1332ba385bb5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_28092213-c991-45a0-ae00-e234b7f2b724" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_461f69f4-e080-486c-9bd3-1332ba385bb5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_eb0d478c-2db2-45f4-af4a-3c7a008aaed5" xlink:href="aqst-20220930.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_28092213-c991-45a0-ae00-e234b7f2b724" xlink:to="loc_aqst_TradeAndOtherReceivablesNet_eb0d478c-2db2-45f4-af4a-3c7a008aaed5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_9643ef93-d616-4c70-9493-ee6566b2c836" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_28092213-c991-45a0-ae00-e234b7f2b724" xlink:to="loc_us-gaap_InventoryNet_9643ef93-d616-4c70-9493-ee6566b2c836" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_2173a676-1ede-4da5-811b-e9f5bcaa5189" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_2173a676-1ede-4da5-811b-e9f5bcaa5189" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_679cb460-b687-466a-93ca-e484d46b5933" xlink:href="aqst-20220930.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_679cb460-b687-466a-93ca-e484d46b5933" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_67d3d0fc-5c9c-428d-9c85-48872ef8ac9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_67d3d0fc-5c9c-428d-9c85-48872ef8ac9f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_5798f9c9-c514-4637-a229-943b91fc80f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_us-gaap_LiabilitiesCurrent_5798f9c9-c514-4637-a229-943b91fc80f5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermLoansPayable_08a1cc82-b861-4efb-a3e0-034552c12ad0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermLoansPayable"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_us-gaap_LongTermLoansPayable_08a1cc82-b861-4efb-a3e0-034552c12ad0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_9eb9f5ff-873f-499a-98b2-90760f3725eb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_6d362050-0d83-4f76-bf33-573f29de5c40" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_9eb9f5ff-873f-499a-98b2-90760f3725eb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_3a285554-236b-4b6c-8b48-920a8de38b28" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_a739b08f-7542-4d0d-a2cd-ecb7ae22014b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3a285554-236b-4b6c-8b48-920a8de38b28" xlink:to="loc_us-gaap_StockholdersEquity_a739b08f-7542-4d0d-a2cd-ecb7ae22014b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_ccec9217-3e63-4d7d-90b0-fac4466a71d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3a285554-236b-4b6c-8b48-920a8de38b28" xlink:to="loc_us-gaap_CommitmentsAndContingencies_ccec9217-3e63-4d7d-90b0-fac4466a71d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_3adc6108-a00d-470a-9db4-827fe2f0333f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3a285554-236b-4b6c-8b48-920a8de38b28" xlink:to="loc_us-gaap_Liabilities_3adc6108-a00d-470a-9db4-827fe2f0333f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="aqst-20220930.xsd#CondensedConsolidatedStatementsofOperationsandComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_00676ffb-ab98-4cbf-83c7-a5be2810f654" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_af821f23-e4e1-4c13-80c9-c8638f863d29" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_00676ffb-ab98-4cbf-83c7-a5be2810f654" xlink:to="loc_us-gaap_NetIncomeLoss_af821f23-e4e1-4c13-80c9-c8638f863d29" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_286e85b8-d4cc-4ca7-9b49-ca9b86dd4829" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_2b0fb096-4e72-4882-98de-1870c752b33a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_286e85b8-d4cc-4ca7-9b49-ca9b86dd4829" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_2b0fb096-4e72-4882-98de-1870c752b33a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_b4583561-ae82-4780-a4f4-55072ed21284" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_286e85b8-d4cc-4ca7-9b49-ca9b86dd4829" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_b4583561-ae82-4780-a4f4-55072ed21284" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_4be87001-12eb-4109-a1d6-d7db5f61d0ed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_286e85b8-d4cc-4ca7-9b49-ca9b86dd4829" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_4be87001-12eb-4109-a1d6-d7db5f61d0ed" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_512d0978-bb25-493c-9855-4bd703ee42b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_30c3d56b-bb98-4432-8284-ce89ef3d863b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_512d0978-bb25-493c-9855-4bd703ee42b3" xlink:to="loc_us-gaap_CostsAndExpenses_30c3d56b-bb98-4432-8284-ce89ef3d863b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_718b9be3-c378-415e-81cd-09259dd0cccc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_512d0978-bb25-493c-9855-4bd703ee42b3" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_718b9be3-c378-415e-81cd-09259dd0cccc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f902c420-9a9c-4636-9389-671f2cf5e0a2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_209c8090-f8fa-4562-bf7d-2bb595e7a000" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestExpense"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f902c420-9a9c-4636-9389-671f2cf5e0a2" xlink:to="loc_us-gaap_InterestExpense_209c8090-f8fa-4562-bf7d-2bb595e7a000" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenues_bac0b7e6-b475-43b8-afd4-4fa44da5c684" xlink:href="aqst-20220930.xsd#aqst_InterestExpenseSaleOfFutureRevenues"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f902c420-9a9c-4636-9389-671f2cf5e0a2" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenues_bac0b7e6-b475-43b8-afd4-4fa44da5c684" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestAndOtherIncomeNet_f81b8a92-348c-49c4-8af7-58afc131f197" xlink:href="aqst-20220930.xsd#aqst_InterestAndOtherIncomeNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f902c420-9a9c-4636-9389-671f2cf5e0a2" xlink:to="loc_aqst_InterestAndOtherIncomeNet_f81b8a92-348c-49c4-8af7-58afc131f197" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_ac443aa0-7791-430e-b0c7-ff03d732029d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f902c420-9a9c-4636-9389-671f2cf5e0a2" xlink:to="loc_us-gaap_OperatingIncomeLoss_ac443aa0-7791-430e-b0c7-ff03d732029d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_5d56ba01-f522-478a-b85f-23f636798f0d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_a6fe61cd-d3ba-4173-a875-e270d268b115" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_5d56ba01-f522-478a-b85f-23f636798f0d" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_a6fe61cd-d3ba-4173-a875-e270d268b115" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_07c5d460-bd84-49bb-b237-ee56a520b0df" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_5d56ba01-f522-478a-b85f-23f636798f0d" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_07c5d460-bd84-49bb-b237-ee56a520b0df" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="aqst-20220930.xsd#CondensedConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_fb45571f-e566-4c9c-ad05-fbd2335f8dd7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_94908fb1-728f-4a98-a0fb-8c017690f8ee" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_fb45571f-e566-4c9c-ad05-fbd2335f8dd7" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_94908fb1-728f-4a98-a0fb-8c017690f8ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_1dc43163-4c8a-4114-a399-3ab134da005f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_fb45571f-e566-4c9c-ad05-fbd2335f8dd7" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_1dc43163-4c8a-4114-a399-3ab134da005f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_792d9d10-047a-4a60-88fa-9cc5a6b62799" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_fb45571f-e566-4c9c-ad05-fbd2335f8dd7" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_792d9d10-047a-4a60-88fa-9cc5a6b62799" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_cd7a68a7-bacb-4205-b588-5bb3d2adeecd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireIntangibleAssets_6524261b-1cc5-4aa9-b7a0-3bf8151c1387" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireIntangibleAssets"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_cd7a68a7-bacb-4205-b588-5bb3d2adeecd" xlink:to="loc_us-gaap_PaymentsToAcquireIntangibleAssets_6524261b-1cc5-4aa9-b7a0-3bf8151c1387" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_4aed2d3d-2b12-4fe7-aee4-fb80459162f3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_cd7a68a7-bacb-4205-b588-5bb3d2adeecd" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_4aed2d3d-2b12-4fe7-aee4-fb80459162f3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuancePrivateOffering_21a9a51c-cbae-4c3a-a3d9-1cb17f5962e1" xlink:href="aqst-20220930.xsd#aqst_ProceedsFromIssuancePrivateOffering"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_aqst_ProceedsFromIssuancePrivateOffering_21a9a51c-cbae-4c3a-a3d9-1cb17f5962e1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_97ebd305-2278-424d-95f8-6fa28f40f3a9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_97ebd305-2278-424d-95f8-6fa28f40f3a9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_9aad1ac6-2a8f-4691-89c4-fcc3cac63967" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_9aad1ac6-2a8f-4691-89c4-fcc3cac63967" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuanceAndExerciseOfWarrants_300ce16c-12da-48bd-bebb-adf6bcba728f" xlink:href="aqst-20220930.xsd#aqst_ProceedsFromIssuanceAndExerciseOfWarrants"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_aqst_ProceedsFromIssuanceAndExerciseOfWarrants_300ce16c-12da-48bd-bebb-adf6bcba728f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_8ab0a9aa-9e23-4eef-ba93-d94353fc3cd3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfDebtExtinguishmentCosts"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_8ab0a9aa-9e23-4eef-ba93-d94353fc3cd3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_b25019dd-d180-4f32-a509-faec27fac8b8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceInitialPublicOffering"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_e9ee5867-1003-4198-8ad4-03d24b9d7a84" xlink:to="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_b25019dd-d180-4f32-a509-faec27fac8b8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInFinanceReceivables_7ac69fbf-d51a-43d9-bbb2-3a3f51bd40c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInFinanceReceivables"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInFinanceReceivables_7ac69fbf-d51a-43d9-bbb2-3a3f51bd40c0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a911ac6f-005f-4c27-9ed6-488ac2c52196" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a911ac6f-005f-4c27-9ed6-488ac2c52196" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_5d39944a-f5b9-4d01-bb33-73973eb2eda6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_DepreciationAndAmortization_5d39944a-f5b9-4d01-bb33-73973eb2eda6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_f1a165d3-4cdb-480c-89a7-d0683d12a524" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_f1a165d3-4cdb-480c-89a7-d0683d12a524" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_80fc7934-a38c-44f1-9220-e0c3aa359615" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_80fc7934-a38c-44f1-9220-e0c3aa359615" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_c981e3a5-64f5-4be3-8d05-b80c71e4f83c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_c981e3a5-64f5-4be3-8d05-b80c71e4f83c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_29bf6929-eb35-45ad-9585-b22f23d55890" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_29bf6929-eb35-45ad-9585-b22f23d55890" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_8328412f-f6e6-48ac-8e36-3ecbe2ac298a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_8328412f-f6e6-48ac-8e36-3ecbe2ac298a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_c8a8da48-9de9-4677-8f35-ddca2fda7b8f" xlink:href="aqst-20220930.xsd#aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_c8a8da48-9de9-4677-8f35-ddca2fda7b8f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_4cc6a0e7-a06c-452c-a9fe-d2f7e2bce6bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_ShareBasedCompensation_4cc6a0e7-a06c-452c-a9fe-d2f7e2bce6bd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_e5dbe3a1-8dbb-401d-acc9-af889fa2b9d8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_NetIncomeLoss_e5dbe3a1-8dbb-401d-acc9-af889fa2b9d8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_e40a02bb-b638-4761-9325-2fefbf59cba2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables"/>
    <link:calculationArc order="12" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_e40a02bb-b638-4761-9325-2fefbf59cba2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_9cd0e155-a33c-449e-a419-98e6b460cb2e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:calculationArc order="13" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_bdb30b53-e813-455d-b412-9766054eedc5" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_9cd0e155-a33c-449e-a419-98e6b460cb2e" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_ad2767aa-41b9-43f5-b43e-3fbb6b06e07c" xlink:href="aqst-20220930.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_36c44621-eb8f-4a3c-a039-78c1bce0a13a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_ad2767aa-41b9-43f5-b43e-3fbb6b06e07c" xlink:to="loc_us-gaap_AccountsReceivableGross_36c44621-eb8f-4a3c-a039-78c1bce0a13a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherReceivablesGrossCurrent_6349f8f1-6b5d-4c8c-aef2-5b2ac2518225" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherReceivablesGrossCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_ad2767aa-41b9-43f5-b43e-3fbb6b06e07c" xlink:to="loc_us-gaap_OtherReceivablesGrossCurrent_6349f8f1-6b5d-4c8c-aef2-5b2ac2518225" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_aff8bff9-3934-4624-a065-daeda4454a9b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_ad2767aa-41b9-43f5-b43e-3fbb6b06e07c" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_aff8bff9-3934-4624-a065-daeda4454a9b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_71d1322a-df34-41c4-a5e0-f678e20f36fd" xlink:href="aqst-20220930.xsd#aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_ad2767aa-41b9-43f5-b43e-3fbb6b06e07c" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_71d1322a-df34-41c4-a5e0-f678e20f36fd" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/InventoriesNetDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#InventoriesNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/InventoriesNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_5a9ffcaa-84f3-4bba-bdf8-9a5d42e43688" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_7130d5d3-b805-492c-a3e7-10329f1c4262" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_5a9ffcaa-84f3-4bba-bdf8-9a5d42e43688" xlink:to="loc_us-gaap_InventoryRawMaterials_7130d5d3-b805-492c-a3e7-10329f1c4262" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InventoryPackingMaterialGross_9619c8fb-a09a-46dc-bc0b-0b054014e0ee" xlink:href="aqst-20220930.xsd#aqst_InventoryPackingMaterialGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_5a9ffcaa-84f3-4bba-bdf8-9a5d42e43688" xlink:to="loc_aqst_InventoryPackingMaterialGross_9619c8fb-a09a-46dc-bc0b-0b054014e0ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_84ca1104-dbb3-4987-8cc9-5f11eb0dd0fe" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_5a9ffcaa-84f3-4bba-bdf8-9a5d42e43688" xlink:to="loc_us-gaap_InventoryFinishedGoods_84ca1104-dbb3-4987-8cc9-5f11eb0dd0fe" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/PropertyandEquipmentNetSummaryofPropertyandEquipmentDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#PropertyandEquipmentNetSummaryofPropertyandEquipmentDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/PropertyandEquipmentNetSummaryofPropertyandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_d25898d7-23fc-4455-8964-471cdec85a2d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_37eb4513-770a-4649-9fc8-d504d9d8b73f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_d25898d7-23fc-4455-8964-471cdec85a2d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_37eb4513-770a-4649-9fc8-d504d9d8b73f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_66770361-4632-492f-9072-5717b8ecaba8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_d25898d7-23fc-4455-8964-471cdec85a2d" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_66770361-4632-492f-9072-5717b8ecaba8" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_e766b4f6-475d-4d57-bd4a-b887bcb15378" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_e766b4f6-475d-4d57-bd4a-b887bcb15378" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c115a007-9c77-4cb0-a85d-b2e56d9fe6ba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c115a007-9c77-4cb0-a85d-b2e56d9fe6ba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_8ea4cbfc-7ab6-4594-b004-61a70f45c207" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_8ea4cbfc-7ab6-4594-b004-61a70f45c207" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_9554a01e-dc83-4960-88c0-3962bb79f5d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_9554a01e-dc83-4960-88c0-3962bb79f5d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_405a5a12-2570-4eef-8596-74d3c390c61b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_3af5e853-fc31-43d4-83b0-07309d087e80" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_405a5a12-2570-4eef-8596-74d3c390c61b" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="simple" xlink:href="aqst-20220930.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails_1"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_2d4ab1ee-6774-4e38-bcde-01ca649d06e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_47fd40f7-d8d1-4444-990f-d7eea28dc308" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_2d4ab1ee-6774-4e38-bcde-01ca649d06e3" xlink:to="loc_us-gaap_OperatingLeaseLiability_47fd40f7-d8d1-4444-990f-d7eea28dc308" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_24b5c9ea-86b9-4cc6-a32d-d14b1f4bdf26" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_2d4ab1ee-6774-4e38-bcde-01ca649d06e3" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_24b5c9ea-86b9-4cc6-a32d-d14b1f4bdf26" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#IntangibleAssetsNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_befe8e14-cf76-4ecc-8aaa-2f253402bd78" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_b7e59f13-6424-4913-ae3d-dc19eb74d566" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_befe8e14-cf76-4ecc-8aaa-2f253402bd78" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_b7e59f13-6424-4913-ae3d-dc19eb74d566" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_c9a88675-0a6a-4d12-b9aa-a782e8d0ee3e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_befe8e14-cf76-4ecc-8aaa-2f253402bd78" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_c9a88675-0a6a-4d12-b9aa-a782e8d0ee3e" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/OthernoncurrentAssetsSummaryofOtherNoncurrentAssetsDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#OthernoncurrentAssetsSummaryofOtherNoncurrentAssetsDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/OthernoncurrentAssetsSummaryofOtherNoncurrentAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_19a590fd-d713-4b82-8cbc-6dd926b1f27f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_daaa70ad-c520-4683-a444-5aa2de2db4e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsMiscellaneousNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_19a590fd-d713-4b82-8cbc-6dd926b1f27f" xlink:to="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_daaa70ad-c520-4683-a444-5aa2de2db4e3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_CumulativeRoyaltyReceivable_897d75cd-975e-49d3-9b05-9b5991b5a7ce" xlink:href="aqst-20220930.xsd#aqst_CumulativeRoyaltyReceivable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_19a590fd-d713-4b82-8cbc-6dd926b1f27f" xlink:to="loc_aqst_CumulativeRoyaltyReceivable_897d75cd-975e-49d3-9b05-9b5991b5a7ce" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#AccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_d9c6c0ee-862b-4e09-80a9-90ea3f6df56c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_8a382412-16bc-439f-9d57-148dc17e088d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_d9c6c0ee-862b-4e09-80a9-90ea3f6df56c" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_8a382412-16bc-439f-9d57-148dc17e088d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_d854dcc3-3a37-4037-b78c-1f3f378a3328" xlink:href="aqst-20220930.xsd#aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_d9c6c0ee-862b-4e09-80a9-90ea3f6df56c" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_d854dcc3-3a37-4037-b78c-1f3f378a3328" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_f70b2883-9d62-4098-8078-a885b6cc2c07" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_d9c6c0ee-862b-4e09-80a9-90ea3f6df56c" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_f70b2883-9d62-4098-8078-a885b6cc2c07" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_e52f62d7-84ce-4780-ba04-155bc048a34e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_d9c6c0ee-862b-4e09-80a9-90ea3f6df56c" xlink:to="loc_us-gaap_TaxesPayableCurrent_e52f62d7-84ce-4780-ba04-155bc048a34e" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220930.xsd#A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_a169a385-c7fc-4b03-a3ff-b7d65eec907a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebt"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_c8b49d4a-5467-4349-b778-55d51fbf2359" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_a169a385-c7fc-4b03-a3ff-b7d65eec907a" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_c8b49d4a-5467-4349-b778-55d51fbf2359" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_811d893c-af2b-4156-b9ac-fa5590119c8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_a169a385-c7fc-4b03-a3ff-b7d65eec907a" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_811d893c-af2b-4156-b9ac-fa5590119c8a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_3b4952f9-0fec-4d6c-9cda-4dce766255c8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_a169a385-c7fc-4b03-a3ff-b7d65eec907a" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_3b4952f9-0fec-4d6c-9cda-4dce766255c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_d166f105-694c-4077-afb1-9d8fef189c2f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_a169a385-c7fc-4b03-a3ff-b7d65eec907a" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_d166f105-694c-4077-afb1-9d8fef189c2f" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
