<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:e79cbc24-9b15-41e0-848b-4d5a157ea9b1,g:887fffdb-3232-46f2-9d05-aad9a05bc84a-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedBalanceSheetsUnaudited" xlink:type="simple" xlink:href="aqst-20220331.xsd#CondensedConsolidatedBalanceSheetsUnaudited"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedBalanceSheetsUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_91d024b1-cb8d-4441-8ee9-de2c66153d6c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_us-gaap_AccountsPayableCurrent_91d024b1-cb8d-4441-8ee9-de2c66153d6c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansPayableCurrent_cf921eeb-5041-49a2-a32d-ee6eb982e6a8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansPayableCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_us-gaap_LoansPayableCurrent_cf921eeb-5041-49a2-a32d-ee6eb982e6a8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_3a5300cf-ccec-4990-8e71-870e9ae3eaea" xlink:href="aqst-20220331.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_3a5300cf-ccec-4990-8e71-870e9ae3eaea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_baf638f4-2a55-4562-b3d1-6bcb70eb3a7d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_baf638f4-2a55-4562-b3d1-6bcb70eb3a7d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_df7d9488-6b82-4655-9266-3345f8e154cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_df7d9488-6b82-4655-9266-3345f8e154cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_8c6394a0-6558-4a87-a952-5dd50fecdd05" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_255d03ec-fe8e-4c37-b98e-b199c0676a95" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_8c6394a0-6558-4a87-a952-5dd50fecdd05" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_3d58c922-d04e-4776-9e30-21273fb6e80d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_8c2b94c2-84ae-4be6-96ee-8e6331e9cff2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_3d58c922-d04e-4776-9e30-21273fb6e80d" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_8c2b94c2-84ae-4be6-96ee-8e6331e9cff2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_80ba00f7-7f14-43e5-bae0-719484504c15" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_3d58c922-d04e-4776-9e30-21273fb6e80d" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_80ba00f7-7f14-43e5-bae0-719484504c15" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_b2a9953c-c213-448d-b89c-810cc0ae593a" xlink:href="aqst-20220331.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_3d58c922-d04e-4776-9e30-21273fb6e80d" xlink:to="loc_aqst_TradeAndOtherReceivablesNet_b2a9953c-c213-448d-b89c-810cc0ae593a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_73fc3c31-ad6f-4593-939c-c04db09ed333" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_3d58c922-d04e-4776-9e30-21273fb6e80d" xlink:to="loc_us-gaap_InventoryNet_73fc3c31-ad6f-4593-939c-c04db09ed333" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_3248057c-e655-4aae-8223-c7d84cd5893c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_9fd7e916-18df-4d18-aeca-e2c26df6b640" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3248057c-e655-4aae-8223-c7d84cd5893c" xlink:to="loc_us-gaap_StockholdersEquity_9fd7e916-18df-4d18-aeca-e2c26df6b640" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_433c792e-16de-4b71-b859-a6405d13e43d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3248057c-e655-4aae-8223-c7d84cd5893c" xlink:to="loc_us-gaap_CommitmentsAndContingencies_433c792e-16de-4b71-b859-a6405d13e43d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_c6b411df-baf8-4eb7-aaa4-2a3b19dd7a93" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_3248057c-e655-4aae-8223-c7d84cd5893c" xlink:to="loc_us-gaap_Liabilities_c6b411df-baf8-4eb7-aaa4-2a3b19dd7a93" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_d6dc17ec-8fcb-4cba-b3fd-8500beaf07d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_d6dc17ec-8fcb-4cba-b3fd-8500beaf07d5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_e0f7a978-c12a-4cf6-82e0-32a7b2852f87" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_e0f7a978-c12a-4cf6-82e0-32a7b2852f87" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_aa433d34-3841-4824-bf29-3eeeff1bdce4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:to="loc_us-gaap_AssetsCurrent_aa433d34-3841-4824-bf29-3eeeff1bdce4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_43ea9f0b-7bd9-482e-a53f-556a7fdd7c31" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_43ea9f0b-7bd9-482e-a53f-556a7fdd7c31" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_fed7c7b6-9834-4aaa-a31c-d267c0b56e0c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b7b37b19-3c07-4db2-b12a-c2850260543b" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_fed7c7b6-9834-4aaa-a31c-d267c0b56e0c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_b2441f87-d440-4ee4-a77a-bda610eef782" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_6cf24c2e-b1b1-4ec1-b43c-dc13b748afcd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_b2441f87-d440-4ee4-a77a-bda610eef782" xlink:to="loc_us-gaap_CommonStockValue_6cf24c2e-b1b1-4ec1-b43c-dc13b748afcd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_d5d57d87-d0ea-4d1e-865a-3bffa9695aaa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_b2441f87-d440-4ee4-a77a-bda610eef782" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_d5d57d87-d0ea-4d1e-865a-3bffa9695aaa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_c91960c1-39ab-4876-9a62-858dd3327240" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_b2441f87-d440-4ee4-a77a-bda610eef782" xlink:to="loc_us-gaap_AdditionalPaidInCapital_c91960c1-39ab-4876-9a62-858dd3327240" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_4466c500-a3e2-417a-a28b-55d2e69b1e02" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_4466c500-a3e2-417a-a28b-55d2e69b1e02" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_27a44cdd-08c7-4783-8a9f-b07770cca6fe" xlink:href="aqst-20220331.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_27a44cdd-08c7-4783-8a9f-b07770cca6fe" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_1348b802-54d0-4c19-aa26-636bb90f9249" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_1348b802-54d0-4c19-aa26-636bb90f9249" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_e1dfd655-8944-4ba7-8f01-fb9ab8498ed1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_us-gaap_LiabilitiesCurrent_e1dfd655-8944-4ba7-8f01-fb9ab8498ed1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermLoansPayable_16930836-81f0-4866-a1f5-547202d364b2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermLoansPayable"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_us-gaap_LongTermLoansPayable_16930836-81f0-4866-a1f5-547202d364b2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_6d7b3d08-389a-4ccd-bc6b-f29afe8266b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_0ac68d5b-d622-47e9-b392-04d79d990324" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_6d7b3d08-389a-4ccd-bc6b-f29afe8266b8" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLossUnaudited" xlink:type="simple" xlink:href="aqst-20220331.xsd#CondensedConsolidatedStatementsofOperationsandComprehensiveLossUnaudited"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLossUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_a746dd2d-c003-4187-9014-6f912e08cdbf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CostsAndExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_598ced64-df1b-4400-8b5b-9f57ace7c25f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_a746dd2d-c003-4187-9014-6f912e08cdbf" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_598ced64-df1b-4400-8b5b-9f57ace7c25f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_81604112-dd7f-43b9-9996-d372c85eba3a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_a746dd2d-c003-4187-9014-6f912e08cdbf" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_81604112-dd7f-43b9-9996-d372c85eba3a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_3704345f-f410-497f-9443-66befd802326" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_a746dd2d-c003-4187-9014-6f912e08cdbf" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_3704345f-f410-497f-9443-66befd802326" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_f074698e-0da0-47c0-a88e-5bff42763b63" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_35b63e4d-2192-4abe-8653-90072ae148a8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CostsAndExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_f074698e-0da0-47c0-a88e-5bff42763b63" xlink:to="loc_us-gaap_CostsAndExpenses_35b63e4d-2192-4abe-8653-90072ae148a8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_894309f7-5b4c-4171-9c01-600dc7a92fc7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_f074698e-0da0-47c0-a88e-5bff42763b63" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_894309f7-5b4c-4171-9c01-600dc7a92fc7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_4c2bd8aa-c655-4a33-998f-be2e64fc2690" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_a2af6fcb-57b6-41e1-9add-af6300e4a3f0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_4c2bd8aa-c655-4a33-998f-be2e64fc2690" xlink:to="loc_us-gaap_NetIncomeLoss_a2af6fcb-57b6-41e1-9add-af6300e4a3f0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f657783d-6196-41c1-8f97-a3e4a6daab6e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_0ba68d77-6e58-42ce-9711-15add4a75d82" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpense"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f657783d-6196-41c1-8f97-a3e4a6daab6e" xlink:to="loc_us-gaap_InterestExpense_0ba68d77-6e58-42ce-9711-15add4a75d82" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenues_4cb80203-adc0-425c-ab84-31a1d2f8eef5" xlink:href="aqst-20220331.xsd#aqst_InterestExpenseSaleOfFutureRevenues"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f657783d-6196-41c1-8f97-a3e4a6daab6e" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenues_4cb80203-adc0-425c-ab84-31a1d2f8eef5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestAndOtherIncomeNet_92a99159-983d-4ffe-997b-1576ea58a295" xlink:href="aqst-20220331.xsd#aqst_InterestAndOtherIncomeNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f657783d-6196-41c1-8f97-a3e4a6daab6e" xlink:to="loc_aqst_InterestAndOtherIncomeNet_92a99159-983d-4ffe-997b-1576ea58a295" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_bb7d0bda-cb76-4e54-a5dd-d5d678de9e9a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f657783d-6196-41c1-8f97-a3e4a6daab6e" xlink:to="loc_us-gaap_OperatingIncomeLoss_bb7d0bda-cb76-4e54-a5dd-d5d678de9e9a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_76b42a3f-c648-4f17-acbf-d632d1e29dc3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_3e495bff-cb50-4cf2-858b-c0624c2d7535" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_76b42a3f-c648-4f17-acbf-d632d1e29dc3" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_3e495bff-cb50-4cf2-858b-c0624c2d7535" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_b0e8129f-3eac-43f9-8c7d-15054054e39a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_76b42a3f-c648-4f17-acbf-d632d1e29dc3" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_b0e8129f-3eac-43f9-8c7d-15054054e39a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlowsUnaudited" xlink:type="simple" xlink:href="aqst-20220331.xsd#CondensedConsolidatedStatementsofCashFlowsUnaudited"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlowsUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_d08b5ac4-a529-4258-bb31-82eaa13dfd8d" xlink:href="aqst-20220331.xsd#aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_d08b5ac4-a529-4258-bb31-82eaa13dfd8d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_99e9559c-b3d8-4ccc-8ed0-7230acf82f95" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_ShareBasedCompensation_99e9559c-b3d8-4ccc-8ed0-7230acf82f95" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_7239dcba-008e-4e27-b46f-9f824d5932a4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_7239dcba-008e-4e27-b46f-9f824d5932a4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_76ff1268-3a45-45cd-970d-89b2097ac0b5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_76ff1268-3a45-45cd-970d-89b2097ac0b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_047819e7-52b2-4590-92d8-f729f4806cd2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_047819e7-52b2-4590-92d8-f729f4806cd2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_618ed44b-d852-4b48-b7de-3e8a0a04196a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_618ed44b-d852-4b48-b7de-3e8a0a04196a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a90b3af6-0f9f-4e28-b664-e75a053e5c25" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a90b3af6-0f9f-4e28-b664-e75a053e5c25" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_ccd3a2b4-0b16-4888-9c6a-786b4fcb93d4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_ccd3a2b4-0b16-4888-9c6a-786b4fcb93d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_11902140-9ea5-4619-be5e-d318f4768017" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_DepreciationAndAmortization_11902140-9ea5-4619-be5e-d318f4768017" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_d9635c93-1555-4308-88bb-363e2e8cc883" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:calculationArc order="10" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_d9635c93-1555-4308-88bb-363e2e8cc883" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_63ad6e7c-d2a1-468e-ada5-ba96b56cbf68" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_63ad6e7c-d2a1-468e-ada5-ba96b56cbf68" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_5f0e27d2-a398-4460-bce1-76388cd237c8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_1f1abf31-f19c-43f9-8efc-6f24f57341df" xlink:to="loc_us-gaap_NetIncomeLoss_5f0e27d2-a398-4460-bce1-76388cd237c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_34ac38c3-8570-4cff-8c78-4a1051965752" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_3eaa960b-6fb6-4f27-be19-269b44faad3a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceInitialPublicOffering"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_34ac38c3-8570-4cff-8c78-4a1051965752" xlink:to="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_3eaa960b-6fb6-4f27-be19-269b44faad3a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_22421df8-d7d5-46c7-a68c-cf29a7d9d0ae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_f25e13a6-fee8-4e13-a6be-ad6fb23c8fa3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_22421df8-d7d5-46c7-a68c-cf29a7d9d0ae" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_f25e13a6-fee8-4e13-a6be-ad6fb23c8fa3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3aa99986-2c79-4c3f-a728-cdb886c542f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_cc098a1e-7c51-459b-b70a-e045407571cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3aa99986-2c79-4c3f-a728-cdb886c542f8" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_cc098a1e-7c51-459b-b70a-e045407571cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_680682be-a1bd-478f-83e0-3e7460a14738" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3aa99986-2c79-4c3f-a728-cdb886c542f8" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_680682be-a1bd-478f-83e0-3e7460a14738" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_dee5d62f-a051-4a20-bd92-fc23ec530b0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3aa99986-2c79-4c3f-a728-cdb886c542f8" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_dee5d62f-a051-4a20-bd92-fc23ec530b0d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandotherreceivablesnetDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#RevenuesandTradeReceivablesNetTradeandotherreceivablesnetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandotherreceivablesnetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_21cd1630-edbf-4f4c-9c70-142d955c33a6" xlink:href="aqst-20220331.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_d9012500-7cbe-4a8c-a898-2da4a287495f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_21cd1630-edbf-4f4c-9c70-142d955c33a6" xlink:to="loc_us-gaap_AccountsReceivableGross_d9012500-7cbe-4a8c-a898-2da4a287495f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherReceivablesGrossCurrent_d70707b9-f11c-47c4-8404-6ebb68d10b31" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherReceivablesGrossCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_21cd1630-edbf-4f4c-9c70-142d955c33a6" xlink:to="loc_us-gaap_OtherReceivablesGrossCurrent_d70707b9-f11c-47c4-8404-6ebb68d10b31" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_7cdefc31-f813-45b5-9a2b-f66213be47cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_21cd1630-edbf-4f4c-9c70-142d955c33a6" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_7cdefc31-f813-45b5-9a2b-f66213be47cb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_72b7ae4d-4f6a-4f1c-81f1-93d675bfae61" xlink:href="aqst-20220331.xsd#aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_21cd1630-edbf-4f4c-9c70-142d955c33a6" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_72b7ae4d-4f6a-4f1c-81f1-93d675bfae61" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/InventoriesNetDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#InventoriesNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/InventoriesNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_6445f36e-75e9-4b63-902b-ab62848c2f82" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InventoryNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_ce0195e2-33d9-4785-9f63-fdd1ed2c542b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_6445f36e-75e9-4b63-902b-ab62848c2f82" xlink:to="loc_us-gaap_InventoryRawMaterials_ce0195e2-33d9-4785-9f63-fdd1ed2c542b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InventoryPackingMaterialGross_ae98d13d-756e-440a-b580-121dca2c0227" xlink:href="aqst-20220331.xsd#aqst_InventoryPackingMaterialGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_6445f36e-75e9-4b63-902b-ab62848c2f82" xlink:to="loc_aqst_InventoryPackingMaterialGross_ae98d13d-756e-440a-b580-121dca2c0227" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_7b9e7473-5f4d-42f5-8730-e5a00e7c4d62" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_6445f36e-75e9-4b63-902b-ab62848c2f82" xlink:to="loc_us-gaap_InventoryFinishedGoods_7b9e7473-5f4d-42f5-8730-e5a00e7c4d62" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#PropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_7382ba29-c9bb-4848-ba35-5463b8504505" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_86f82c03-d1e0-43bf-8327-4ecfed198421" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_7382ba29-c9bb-4848-ba35-5463b8504505" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_86f82c03-d1e0-43bf-8327-4ecfed198421" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_7075ab65-f88e-45cd-a9eb-d885c3044a8a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_7382ba29-c9bb-4848-ba35-5463b8504505" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_7075ab65-f88e-45cd-a9eb-d885c3044a8a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6d13d778-6e34-439f-af40-77d330eb3d1a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_14e500a7-74a2-4677-98c3-9195868f2480" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6d13d778-6e34-439f-af40-77d330eb3d1a" xlink:to="loc_us-gaap_OperatingLeaseLiability_14e500a7-74a2-4677-98c3-9195868f2480" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_5f143519-d8dd-4d9f-8652-4427f17829b4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_6d13d778-6e34-439f-af40-77d330eb3d1a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_5f143519-d8dd-4d9f-8652-4427f17829b4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="simple" xlink:href="aqst-20220331.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails_1"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_e8f5a839-aa4d-44bc-b0f8-10293c721eb0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_e8f5a839-aa4d-44bc-b0f8-10293c721eb0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_5680d982-100b-4ed4-90cf-3a896697f966" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_5680d982-100b-4ed4-90cf-3a896697f966" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_514eeb4b-553b-43b6-9de1-4c9f3f81fbf2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_514eeb4b-553b-43b6-9de1-4c9f3f81fbf2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_7f84ebdd-2c79-4c87-ba23-846e594d2bf1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_7f84ebdd-2c79-4c87-ba23-846e594d2bf1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_2c4431a3-a7bb-4bd1-8f8c-b53aa3e30388" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4beba5c0-364a-4a05-8daa-5d0bda11b269" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_2c4431a3-a7bb-4bd1-8f8c-b53aa3e30388" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#IntangibleAssetsNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_a33d35dd-a70e-49ba-a50a-bbf8bb14bc11" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_993a0019-c822-48df-acd5-4c378fde4716" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_a33d35dd-a70e-49ba-a50a-bbf8bb14bc11" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_993a0019-c822-48df-acd5-4c378fde4716" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_ad95bb4b-c5db-4205-bb3a-789ab9895c82" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_a33d35dd-a70e-49ba-a50a-bbf8bb14bc11" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_ad95bb4b-c5db-4205-bb3a-789ab9895c82" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/OthernoncurrentAssetsDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#OthernoncurrentAssetsDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/OthernoncurrentAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_0180ea9d-0658-4c9c-bdad-9c45e26c0413" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_1390df84-0b87-436a-a477-2c216e41b609" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsMiscellaneousNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_0180ea9d-0658-4c9c-bdad-9c45e26c0413" xlink:to="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_1390df84-0b87-436a-a477-2c216e41b609" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_CumulativeRoyaltyReceivable_aa68c47a-3759-4c17-8a8e-8cead1fd52d1" xlink:href="aqst-20220331.xsd#aqst_CumulativeRoyaltyReceivable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_0180ea9d-0658-4c9c-bdad-9c45e26c0413" xlink:to="loc_aqst_CumulativeRoyaltyReceivable_aa68c47a-3759-4c17-8a8e-8cead1fd52d1" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#AccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_1ec127f4-70dd-4276-9076-b3212784391a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_370c4c36-fe7a-43a6-b42f-c1d5def528d4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_1ec127f4-70dd-4276-9076-b3212784391a" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_370c4c36-fe7a-43a6-b42f-c1d5def528d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_76afac17-bced-45bf-bd31-e2772664f3b5" xlink:href="aqst-20220331.xsd#aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_1ec127f4-70dd-4276-9076-b3212784391a" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_76afac17-bced-45bf-bd31-e2772664f3b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_6547e34b-3873-45a5-b60e-622fd55558cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_1ec127f4-70dd-4276-9076-b3212784391a" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_6547e34b-3873-45a5-b60e-622fd55558cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_7c616484-ed62-4421-95d6-9ec16beb4018" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_1ec127f4-70dd-4276-9076-b3212784391a" xlink:to="loc_us-gaap_TaxesPayableCurrent_7c616484-ed62-4421-95d6-9ec16beb4018" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220331.xsd#A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_c99322f4-f914-49d4-a4c1-5614a732ed76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_25b52b61-b54b-42df-9d60-7e4cc74a7708" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_c99322f4-f914-49d4-a4c1-5614a732ed76" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_25b52b61-b54b-42df-9d60-7e4cc74a7708" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_61b7c3cd-8f31-4eee-b1a7-f393fda3b75f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_c99322f4-f914-49d4-a4c1-5614a732ed76" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_61b7c3cd-8f31-4eee-b1a7-f393fda3b75f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_31191e28-bf83-4d98-97e1-06fb287ec4fa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_c99322f4-f914-49d4-a4c1-5614a732ed76" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_31191e28-bf83-4d98-97e1-06fb287ec4fa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_8c245b12-b60d-45d8-95c0-a99a59ada77d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_c99322f4-f914-49d4-a4c1-5614a732ed76" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_8c245b12-b60d-45d8-95c0-a99a59ada77d" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
